The number of malaria parasites in your blood can change each day. 2. July 13, 2019 Nisha Rijal Lab Diagnosis of Parasitic Disease, Parasitology, Staining techniques in Microbiology 3. The staining procedures listed in this insert have given satisfactory results in our laboratory. The procedure follows these steps: collection of peripheral blood, staining of smear with Giemsa stain and examination of red blood cells for malaria parasites under the microscope. A phlebotomist, a person specifically trained to draw blood, first … Preparation of thick and thin blood smears, appropriate staining procedure and detection and identification of hemo-parasites are crucial to clinical diagnosis of many parasitic diseases.
Procedure for Malaria, Direct Smear Test Blood smears are taken most often from a finger prick. In thin films the red blood cells are fixed and parasitized cells can be identified by their morphology. A6592; A6600 Description Field's stain is a histological method for staining of blood smears. Thick smear . Positive control slides should be included in each run. Individual color preferences may necessitate slight adjustments to the times utilized. On properly prepared smears hemo-parasites can be identified on the basis of their morphologic features. The definitive diagnosis of malaria infection and other parasites is still based on finding such organisms in blood films. These include species of malaria, trypanosomes, babesias and microfilariae of filarial nematodes. Giemsa Stain: Principle, Procedure and Results. So your test might say you don’t have malaria even if you do. 3.
Staining Procedure 2: Thick Film Staining Add a thick smear of blood and air dry for 1 hour on a staining rack. It is not fixed in methanol; this allows the red blood cells to be hemolyzed, and leukocytes and any malaria parasites present will be the only detectable elements. Dip the thick blood smear into diluted Giemsa stain (prepared by taking 1ml of the stock solution and adding to 49ml of phosphate buffer or distilled water, but the results may vary differently). Field’s stain A & Field’s stain B Product No. Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution.
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